1. Her reaction to your questions : What does she say when you ask her if she is a virgin? In most cases, (if the girl is not a feminist), she would react calmly or shyly. The one who has lost it, may get irritated by your inquiries or angry with you. She has it no more and those questions make her nervous.
2. She gets engaged in sex talk easily: A v!rgin girl has a bit of this natural modesty. She does not have firsthand experience in the process, so sex talk makes her uncomfortable. She would not be easily engaged in it.
3. How she conducts herself with men: V!rginity loss is a serious step in every woman’s life. It involves some big changes in her behavior as well. But once there is not more to lose, a girl may start acting more lavishly and self-indulging with men. If she is a student, she might be hanging out in the teachers’ office all the time.